作者： Zhang, Yue-Fei;Tang, Jian;Li, Tao;Liu, Yun;Li, Yan*

期刊： Polymer Bulletin,2022年79(2):1057-1068 ISSN：0170-0839

通讯作者： Li, Yan

作者机构： [Tang, Jian; Li, Tao; Zhang, Yue-Fei; Li, Yan; Liu, Yun] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

通讯机构： [Li, Yan] C;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

关键词： Polyacrolein;Fluorescein;Atom transfer radical polymerization

摘要： In this paper, the polymerization of acrolein (A) via UV-mediated atom transfer radical polymerization (ATRP) is reported. The optimization of the experimental conditions of the polymerization is investigated, and it shows that dimethyl sulfoxide as solvent, ethyl 2-bromoisobutyrate (EBIB) and fluorescein (FL) as catalyst, and [A]0/[EBIB]0/[FL]0 = 200/1/0.1 in the period of 5 h at 47 °C are suitable conditions for the reaction. In this way, the yield of the polymer is 24.5%. The glass transition temperature and melting point of polyacrolein characterized by differential scanning calorimetry are 115.5 °C and 165.7 °C, respectively. At low conversion, the polymerization conforms to be the first-order kinetics reaction. The dependence of polymerization on light source is proved by “on/off” light source experiment. In short, this study opens up a new way for the ATRP of acrolein, and the polyacrolein with abundant aldehyde groups can be used in the fields of biomedical labeling, immobilization carrier and adsorption of organic amines. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.

语种： 英文

作者： Li, Yan;Li, Tao;Tang, Jian;Liu, Yun;Huang, Pengmian;...

期刊： Macromolecular Reaction Engineering,2021年15(1) ISSN：1862-832X

通讯作者： Zhang, Yue-Fei

作者机构： [Li, Tao; Tang, Jian; Zhang, Yue-Fei; Li, Yan; Liu, Yun; Huang, Pengmian] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

通讯机构： [Zhang, Yue-Fei] C;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

关键词： Aldehydes;Atom transfer radical polymerization;Free radicals;Herbicides;Infrared spectroscopy;Mass spectrometry;Monomers;Nuclear magnetic resonance spectroscopy;Free radical initiators;Matrix assisted laser desorption ionization;Monomer concentration;Polymerization reaction;Reaction temperature;Structure characterization;Structure of the polymers;Synthesis and characterizations;Free radical polymerization

摘要： A radical polymerization reaction of acrolein is reported in this article. The free radical initiator which can effectively promote the free radical polymerization of acrolein is screened out. The optimal conditions of the reaction are investigated and the yield could be up to 93.67%, in which the ratio of initiator to monomer is 1:50, monomer concentration is 7.5molL−1, reaction temperature is 50°C, and the reaction time is 6h. The structure characterizations of the obtained polymers are performed using hydrogen nuclear magnetic resonance spectroscopy, fouriertransform infrared spectroscopy, and matrix-assisted laser desorption ionization time of fligh mass spectroscopy. The results show that the structure of the polymer contains fragments generated by decomposition of the initiator, aldehyde groups, and vinyl groups. The reaction mechanism of acrolein polymerization in the presence of free radical initiator is proposed. Thus, a novel method for the preparation of polyacrolein via radical polymerization is provided in this article. © 2020 Wiley-VCH GmbH

语种： 英文

作者： Tang, Jian;Zhang, Yue-Fei;Liu, Yun;Li, Yan*;Hu, Hui

期刊： Separation and Purification Technology,2020年252:117445 ISSN：1383-5866

通讯作者： Li, Yan

作者机构： [Tang, Jian; Zhang, Yue-Fei; Li, Yan; Liu, Yun] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.;[Hu, Hui] Fuzhou Univ, Sch Chem Engn, Fuzhou 350116, Peoples R China.

通讯机构： [Li, Yan] C;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

关键词： Congo red;Polyacrolein;Reusability;Adsorption

摘要： Polyacrolein (PA) was prepared by photoinduced free radical polymerization for adsorption of congo red (CR). The structural and physicochemical properties of PA were characterized using BET, zeta potential measure-ments, SEM, FT-IR and XPS. The adsorption process of CR accorded with the pseudo-second-order kinetics, while the adsorption isotherm conformed to be the Langmuir model and the adsorption was proved to be the spon-taneous and endothermic process (Delta G(0) < 0, Delta H-0 > 0). Electrostatic attraction, hydrogen bond and chemical reaction (formation of imine) were the critical driving forces of adsorption. The good removal efficiency ob-tained during the five executed cycles suggested a good reusability of PA. It was noteworthy that salts in CR solution could promote the adsorption capacity of PA, in which Ca (II) could obviously improve the adsorption capacity and shorten the time to reach adsorption equilibrium. These results have provided very useful in-formation for removing CR and enhanced our understanding of the adsorption mechanism of PA on CR.

语种： 英文

作者： Guo, Bogeng;Hou, Linxi*;Li, Yan;Xiao, Longqiang*

期刊： Polymer,2020年189:122201 ISSN：0032-3861

通讯作者： Hou, Linxi;Xiao, Longqiang

作者机构： [Hou, Linxi; Xiao, Longqiang; Guo, Bogeng] Fuzhou Univ, Coll Chem Engn, Fuzhou 350108, Peoples R China.;[Li, Yan] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Peoples R China.

通讯机构： [Hou, LX; Xiao, LQ] F;Fuzhou Univ, Coll Chem Engn, Fuzhou 350108, Peoples R China.

关键词： Halogen exchange;Organocatalyzed;Controlled radical polymerization

摘要： Alkyl bromide (R-Br) was employed as a starting compound (precursor) to initiate the bulk controlled radical polymerization of methyl methacrylate (MMA) under thermal condition (60 degrees C) in the presence of tetra-noctylammonium iodide (Oct(4)NI) and 2,2'-azobisisoheptonitrile (V65). Alkyl iodide (R-I) and tetraoctylammonium bromide (Oct(4)NBr) were in situ generated by the reaction of R-Br with Oct(4)NI and then R-I was employed as an initiating dormant species for the polymerization. Carbon-iodine (C-I) bonds were cleaved and radicals were generated by the catalyst of Oct(4)NI and Oct(4)NBr. The chain end groups of the obtained polymers contain both I and Br, which was proved by the results of H-1 nuclear magnetic resonance spectra, and elemental analysis. The reversible halogen exchange of Br with I at the polymer chain end promotes the polymerization. The introducing of V65 not only increased the polymerization speed, but also ensured high monomer conversion. Low-polydispersity (M-w/M-n = 1.1-1.5) polymers were obtained with high conversions (e.g., 70-90%) in the polymerizations of methyl methacrylate, styrene, acrylonitrile, and functional methacrylates. The attractive feature was that alkyl bromide was able to initiate the controlled radical polymerization of methyl methacrylate under thermal condition in the presence of organic catalyst. Besides, this new system presented desirable features of high monomer versatility and the accessibility to a wide range of polymer structural designs for use in a range of applications.

语种： 英文

作者： Qing, Zhihe*;Li, Yacheng;Li, Younan;Luo, Guoyan;Hu, Jinlei;...

期刊： Microchimica Acta,2020年187(9):497 ISSN：0026-3672

通讯作者： Qing, Zhihe;Yang, Ronghua

作者机构： [Li, Yacheng; Li, Younan; Luo, Guoyan; Zou, Zhen; Lei, Yanli; Yang, Ronghua; Hu, Jinlei; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Cytochem, Changsha 410114, Peoples R China.;[Qing, Zhihe; Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.;[Yang, Ronghua] Hunan Normal Univ, Coll Chem & Chem Engn, Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China.

通讯机构： [Qing, ZH; Yang, RH] C;[Qing, Zhihe] U;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Cytochem, Changsha 410114, Peoples R China.;Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada.

关键词： Gold nanorods;Iodine etching;Colorimetric assay;Organophosphorus pesticides;Triazophos

摘要： For the first time it isdemonstrated that sulfhydryl compounds can suppress longitudinal etching of gold nanorods via consuming oxidizers, which provides a new signaling mechanism for colorimetric sensing. As a proof of concept, a colorimetric assay is developed for detecting organophosphorus pesticides, which are most widely used in modern agriculture to improve food production but with high toxicity to animals and the ecological environment. Triazophos was selected as a model organophosphorus pesticide. In the absence of triazophos, the active acetylcholinesterase can catalyze the conversion of acetylthiocholine iodide to thiocholine whose thiol group can suppress the I(2)-induced etching of gold nanorods. When triazophos is present, the activity of AchE is inhibited, and I(2)-induced etching of gold nanorods results in triazophos concentration-dependent color change from brown to blue, pink, and red. The aspect ratio of gold nanorods reduced with gradually blue-shifted longitudinal absorption. There was a linear detection range from 0 to 117nM (R(2) = 0.9908), the detection limit was 4.69nM, and a good application potential was demonstrated by theassay of real water samples. This method will not only contribute to public monitoring of organophosphorus pesticides but also has verified a new signaling mechanism which will open up a new path to develop colorimetric detection methods. It has been first found that sulfhydryl compounds can suppress longitudinal etching of gold nanorods (AuNRs) via consuming oxidizers, which provides a new signaling mechanism for colorimetric sensing. As a proof of concept, a colorimetric assay is developed for sensitively detecting organophosphorus pesticides (OPs). It will not only contribute to public monitoring of OPs but also has verified a new signaling mechanism which will open up a new path to develop multicolor colorimetric methods.

语种： 英文

作者： Chen, Shiya;Liu, Jin;Li, Yinhui*;Wu, Xu*;Yuan, Quan;...

期刊： TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2020年131:116010 ISSN：0165-9936

通讯作者： Zheng, Jing;Wu, Xu;Li, Yinhui

作者机构： [Yuan, Quan; Zheng, Jing; Liu, Jin; Chen, Shiya] Hunan Univ, Coll Chem & Chem Engn, Inst Chem Biol & Nanomed ICBN, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wu, Xu; Liu, Jin] Cent South Univ, Canc Res Inst, NHC Key Lab Carcinogenesis, Changsha 410083, Peoples R China.;[Wu, Xu; Liu, Jin] Cent South Univ, Canc Res Inst, Chinese Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha 410083, Peoples R China.;[Wu, Xu; Liu, Jin] Cent South Univ, Sch Basic Med Sci, Changsha 410083, Peoples R China.;[Chen, Shiya; Li, Yinhui] Xiangtan Univ, Coll Chem, Key Lab Green Organ Synth & Applicat Hunan Prov, Key Lab Environm Friendly Chem & Applicat,Minist, Xiangtan 411105, Peoples R China.

通讯机构： [Zheng, Jing] H;[Wu, Xu] C;[Li, Yinhui] X;Hunan Univ, Coll Chem & Chem Engn, Inst Chem Biol & Nanomed ICBN, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Cent South Univ, Canc Res Inst, NHC Key Lab Carcinogenesis, Changsha 410083, Peoples R China.

关键词： Cancer therapy;Fluorescent nanoprobe;Hypoxia;Intracellular imaging;Reductase

摘要： With the exaggerated growth of solid tumor, the depletion of O-2 in interior cells far exceeds their blood supply, leading to low levels of O-2 in tumor microenvironment (Hypoxia). Low concentration of O-2 often cause many problems in tumor cells, including poor prognosis, increased metastasis and pro-survival changes in gene expression. Thus, developing new imaging strategy for living cells under hypoxic conditions is strongly desirable. In this review, we discuss recent progress in hypoxia-responsive fluorescent nanoprobes for imaging and cancer therapy. First, we describe two basic elements of fluorescent nanoprobes, including recognition moiety and nanomaterial-based carrier. Then, recent progress in fluorescent nanoprobes for imaging under hypoxic conditions is summarized. Finally, we outline development of hypoxia-responsive fluorescent nanoprobes for simultaneous imaging and cancer treatment. We aim to provide a comprehensive summary of application for distinguishing tumor hypoxic status, diagnosing disease and developing hypoxia-responsive anticancer nanodrugs. (C) 2020 Elsevier B.V. All rights reserved.

语种： 英文

作者： He, Juan;Zhang, Yue-Fei*;Ren, Xiao-Qiong;Li, Yan

期刊： Polymer Bulletin,2019年76(11):5559-5575 ISSN：0170-0839

通讯作者： Zhang, Yue-Fei

作者机构： [Zhang, Yue-Fei; Li, Yan; Ren, Xiao-Qiong; He, Juan] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.

通讯机构： [Zhang, Yue-Fei] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.

关键词： Compound nucleating agents;1,3,5-Benzenetricarboxylic acid tris(cyclohexylamide);Isotactic polypropylene;Nucleation efficiency;Properties

摘要： Three α/β compound nucleating agents based on 1,3,5-benzenetricarboxylic acid tris(cyclohexylamide) (BTA-99) were prepared by using β-nucleating agents aromatic diamides (TMB-5), dicarboxylic acid salts (CaPi) and rare earth compound (WBG-II), and nucleation effects of the nucleating agents in isotactic polypropylene (iPP) were investigated. The optimal ratio of BTA-99 to TMB-5, CaPi and WBG-II was 1:1, 4:1 and 2:3, respectively, and the crystallization peak temperatures of iPP nucleated with BTA-99/TMB-5, BTA-99/CaPi and BTA-99/WBG-II were increased from 117.7 °C of pure iPP to 129.7 °C, 127.6 °C and 128.9 °C under the optimal ratio, respectively. Moreover, all compound nucleating agents with the optimal compound ratio improved both the stiffness and the toughness of iPP, and TMB-5 was more suitable as a component for preparing compound nucleating agents with BTA-99 than WBG-II and CaPi. Meanwhile, an interesting phenomenon was found that the smaller the difference of the nucleation efficiency between two nucleating agents, the better the nucleation effect of the compound agent was.

语种： 英文

作者： Li, Younan;Luo, Guoyan;Qing, Zhihe*;Li, Xiaoxuan;Zou, Zhen;...

期刊： Microchimica Acta,2019年186(8):1-7 ISSN：0026-3672

通讯作者： Qing, Zhihe;Yang, Ronghua

作者机构： [Li, Younan; Li, Xiaoxuan; Luo, Guoyan; Zou, Zhen; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.

通讯机构： [Qing, ZH; Yang, R] C;Changsha Univ Sci & Technol, Sch Chem & Food Engn, Hunan Prov Engn Res Ctr Food Proc Aquat Biot Reso, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.

关键词： amitrole;catalase;gold;herbicide;hydrogen peroxide;iodine;nanotube;chemistry;color;colorimetry;food contamination;limit of detection;metabolism;procedures;water pollutant;Amitrole;Catalase;Color;Colorimetry;Food Contamination;Gold;Herbicides;Hydrogen Peroxide;Iodine;Limit of Detection;Nanotubes;Water Pollutants, Chemical

摘要： A colorimetric and visual assay is described for the herbicide aminotriazole (ATZ). It is based on the etching of gold nanorods (AuNRs) by iodine which is formed on oxidation of iodide via H2O2. Longitudinal etching of the AuNRs occurs quickly and is accompanied by a color change from dark blue to red. In the absence of ATZ and the presence of active catalase (CAT), H2O2 is quickly decomposed into water, and the AuNRs will not be etched. In thepresence of ATZ, CAT is partially deactivated, and this affects the amount of available H2O2 and, consequently, of the iodine. Hence, the color is significantly changed. The color changes can be easily detected with bare eyes. The assay has a linear response in the 5 to 70μM concentration range, with a detection limit of 1.3μM and high selectivity for ATZ. It was applied to the determination of ATZ in water and food samples. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.

语种： 英文

作者： Ren, Xiao-Qiong;Zhang, Yue-Fei*;He, Juan;Li, Yan

期刊： Journal of Thermal Analysis and Calorimetry,2019年135(6):3321-3328 ISSN：1388-6150

通讯作者： Zhang, Yue-Fei

作者机构： [Zhang, Yue-Fei; Li, Yan; Ren, Xiao-Qiong; He, Juan] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, 960 South Wanjiali Rd, Changsha 410114, Hunan, Peoples R China.

通讯机构： [Zhang, Yue-Fei] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, 960 South Wanjiali Rd, Changsha 410114, Hunan, Peoples R China.

关键词： Chlorine compounds;Crystallization;Decomposition;Differential scanning calorimetry;Metals;Nucleation;Salts;Crystallization behavior;Crystallization peak temperatures;Double decomposition;Isotactic poly(propylene) (iPP);Isotactic polypropylene;Metal salt;Nucleation effect;Nucleation efficiency;Polypropylenes

摘要： A series of adipic acid metal salts as nucleating agents for isotactic polypropylene (iPP) were synthesized from adipic acid (Adi) and corresponding metal hydroxides or chlorides by neutralization or double decomposition reaction. The effect of different adipate on the crystallization and melting behavior of iPP were investigated by differential scanning calorimetry (DSC). The results indicated that after adding different metal salts of adipic acids, the crystallization peak temperature of nucleated iPP was increased by different degree. Adi, barium adipate (Adi-Ba), and zinc adipate (Adi-Zn) had higher nucleation efficiency. Notably, Adi-Zn and Adi-Ba were highly effective β-crystal nucleating agents; the others were α-crystal nucleating agents. In addition, the relative content of β-crystals in nucleated iPP was higher than 0.9 with the addition of Adi-Ba or Adi-Zn, especially when Adi-Zn was added at 0.05mass% reached 0.9422, indicating that Adi-Ba and Adi-Zn are highly active and selective β-nucleating agents for iPP. © 2018, Akadémiai Kiadó, Budapest, Hungary.

语种： 英文

作者： Wang, Yingqian;Li, Zhihao;Lin, Qiaosong;Wei, Yurong;Wang, Jie;...

期刊： ACS SENSORS,2019年4(8):2124-2130 ISSN：2379-3694

通讯作者： Yuan, Quan

作者机构： [Wang, Yingqian; Yuan, Quan; Li, Zhihao; Wei, Yurong; Lin, Qiaosong; Li, Yingxue; Wang, Jie] Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.;[Yuan, Quan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Yuan, Quan] W;[Yuan, Quan] H;Wuhan Univ, Coll Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.

关键词： long-lifetime luminescence;photonic crystals;miRNA detection;urine;autofluorescence

摘要： Detection of biomarkers in complex samples is a significant health plan strategy for medical diagnosis, therapy monitoring, and health management. However, high background noise resulting from impurities and other analytes in complex samples has hampered the improvement of detection sensitivity and accuracy. Herein, an ultralow background biochip based on time-gated luminescent probes supported by photonic crystals (PCs) was successfully developed for detection of bladder cancer (BC)-related miRNA biomarkers with high sensitivity and specificity in urine samples. Coupled with the time-gated luminescence of long-lifetime luminescence probes and the luminescence-enhanced capability of PCs, the short-lived autofluorescence can be efficiently removed; thus, the detection sensitivity will be significantly improved. Benefiting from these merits, a detection limit of 26.3 fM is achieved. Furthermore, the biochip exhibits excellent performance in urinary miRNA detection, and good recoveries are also obtained. The developed biochip possesses unique properties of ultralow background and luminescence enhancement, thus offering a suitable tool for the detection of BC-related miRNA in urine. With rational design of probe sequences, the biochip holds great potential for many other biomarkers in real patient samples, making it valuable in areas such as medical diagnosis and disease evaluation.

语种： 英文

作者： Yang, Sheng;Wen, Xidan;Yang, Xiaoguang;Li, Yuan;Guo, Chongchong;...

期刊： Analytical Chemistry,2018年90(24):14514-14520 ISSN：0003-2700

通讯作者： Yang, Ronghua

作者机构： [Wen, Xidan; Yang, Sheng; Yang, Xiaoguang; Guo, Chongchong; Yang, Ronghua; Zhou, Yibo; Li, Heping] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.;[Li, Yuan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Yang, Ronghua] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.

摘要： Febrile seizure (FS), a frequently encountered seizure disorder in pediatric populations, can cause hippocampus damage. It has been elucidated that sulfur dioxide (SO2) content is overproduced during the development of FS and related brain injury. Thus, monitoring in situ the level of endogenous SO2 in FS-related models is helpful to estimate the pathogenesis of FS-induced brain injury, but the effect detection method remains to be explored. Herein, we developed a two-photon energy transfer cassette based on an acedan-anthocyanidin scaffold, TP-Ratio-SO2, allowing us to achieve this purpose. TP-Ratio-SO2 specifically responds to SO2 derivatives (HSO3 -/SO3 2-) in an ultrafast fashion (less than 3 s), and HSO3 -/SO3 2- can be sensitively determined with a detection limit of 26 nM. Moreover, it exhibits significant changes in two well-resolved fluorescence emissions (Δ λ = 140 nm) by reacting with HSO3 -/SO3 2-, behaving as a ratiometric fluorescent sensor. Importantly, ratiometric imaging of endogenous SO2 derivatives generation in hyperpyretic U251 cells and in a rat model of FS-treated hippocampus damage was successfully carried out by TP-Ratio-SO2, demonstrating that it may be a promising tool for studying the role of SO2 in FS-associated neurological diseases. Copyright © 2018 American Chemical Society.

语种： 英文

作者： Li, Yan;Li, Tao;Xiao, Long-Qiang;Zhang, Yue-Fei*

期刊： Industrial & Engineering Chemistry Research,2018年57(39):12987-12992 ISSN：0888-5885

通讯作者： Zhang, Yue-Fei

作者机构： [Li, Tao; Zhang, Yue-Fei; Li, Yan] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Xiao, Long-Qiang] South China Normal Univ, Inst Elect Paper Displays, South China Acad Adv Optoelect, Guangzhou 510631, Guangdong, Peoples R China.

通讯机构： [Zhang, Yue-Fei] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.

摘要： The polycondensation of 2-pyrazolines is presented. The products are oligomers with the five-membered rings of the 2-pyrazoline as the backbone and the reaction is a polycondensation reaction by decomposition of ROH from the hydrogen atom in the -NH- group and the -OR in the ester group. Metal catalysts, reaction periods, and the addition amounts of the catalyst are investigated to optimize the reaction conditions. The products are down-/up-conversion fluorescence molecules that can significantly enhance cell imaging through the synchronized excitation of visible and near-infrared light and may be widely used in other optical fields. © 2018 American Chemical Society.

语种： 英文

作者： Yang, Sheng;Guo, Chongchong;Li, Yuan;Guo, Jingru;Xiao, Jie;...

期刊： ACS SENSORS,2018年3(11):2415-2422 ISSN：2379-3694

通讯作者： Yang, Ronghua

作者机构： [Xiao, Jie; Yang, Sheng; Guo, Chongchong; Guo, Jingru; Li, Jiangsheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Li, Yuan; Yang, Ronghua] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Li, Yuan; Yang, Ronghua] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Yang, Ronghua] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.

关键词： two-photon;FRET;ICT;ratiometric fluorescent probe;cysteine;bioimaging

摘要： The development of an efficient ratiometric two-photon fluorescence imaging probe is crucial for in situ monitoring of biothiol cysteine (Cys) in biosystems, but the current reported intramolecular charge transfer (ICT)-based one suffers from serious overlap between the shifted emission bands. To address this issue, we herein for the first time constructed an ICT-mediated two-photon excited fluorescence resonance energy transfer (TP-FRET) system consisting of a two-photon fluorogen benzo[h]chromene and a Cys-responsive benzoxadiazole-analogue dye. Different from a previous mechanism that utilized single two-photon fluorogen to acquire a ratiometric signal, ICT was used to switch on the TP-FRET process of the energy transfer dyad by eliciting an absorption shift of benzoxadiazole with Cys to modulate the spectral overlap level between benzo[h]chromene emission and benzoxadiazole absorption, resulting in two well-separated emission signal changes with large emission wavelength shift (120 nm), fixed two-photon excitation maximum (750 nm), and significant variation in fluorescence ratio (over 36-fold). Therefore, it can be successfully employed to ratiometrically visualize Cys in HeLa cells and liver tissues. Importantly, this new ICT-mediated TP-FRET integration mechanism would be convenient for designing ratiometric two-photon fluorescent probes with two well-resolved emission spectra suitable for high resolution two-photon fluorescence bioimaging. © 2018 American Chemical Society.

语种： 英文

作者： 李彦;王发祥;俞健

期刊： 当代教育实践与教学研究,2018年(5):316-317 ISSN：2095-6711

作者机构： 长沙理工大学化学与生物工程学院;[王发祥; 李彦; 俞健] 长沙理工大学

关键词： 教学内容改革;教学方法改革;考核模式改革;达成目标

摘要： 基于OBE教育模式的食品科学导论课程改革以学生为中心,以学习成果为目标,通过充实教学内容、更新教学方法和改革考核模式达成目标.教学内容改革包括分环节教学,每个教学课时分成四个环节:线下预习检查、线上线下内容讲解、课堂讨论、视频放送.教学方法改革包括"三型"教学模式、板书多媒体、 网络课程.考核方式改革包括在线问答、分组讨论、课程论文、期末考核等多种综合评定.

语种： 中文

作者： Li, Yuan;Yang, Sheng;Zheng, Jing;Zou, Zhen;Yang, Ronghua*;...

期刊： LANGMUIR,2018年34(49):15028-15044 ISSN：0743-7463

通讯作者： Yang, Ronghua

作者机构： [Li, Yuan; Zheng, Jing; Yang, Ronghua; Tan, Weihong] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Zou, Zhen; Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Yang, Ronghua] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.

关键词： Biocompatibility;Medical nanotechnology;Nanostructured materials;Nanostructures;Clinical trial;Design mechanisms;DNA nanostructures;Nanotheranostics;Programmability;Theranostics;DNA;antineoplastic agent;DNA;drug carrier;nanomaterial;animal;chemistry;conformation;human;mouse;neoplasm;procedures;RAW 264.7 cell line;theranostic nanomedicine;Animals;Antineoplastic Agents;DNA;Drug Carriers;Humans;Mice;Nanostructures;Neoplasms;Nucleic Acid Conformation;RAW 264.7 Cells;Theranostic Nanomedicine

摘要： Nanotheranostics, combing diagnostic and therapeutic components in an all-in-one nanomaterial, possess exciting potentials for precision nanomedicine. However, a major obstacle for current nanotheranostics to enter preclinical and/or clinical trials is the intrinsic toxicities of these nanomaterials. As an emerging biomaterial, the bioinspired DNA nanostructure shows advantages for constructing better nanotheranostics due to its excellent features, including native biocompatibility, full programmability, and ready accessibility. In this feature article, we highlight recent advances in the design of DNA-nanostructure-based diagnostics and/or therapeutics capable of specifically responding to biological stimuli in a dynamic way, with a particular focus on the design mechanism, responsive performance, and potential for preclinical and/or clinical trials in personalized theranostics. © 2018 American Chemical Society.

语种： 英文

作者： Zhou, Yibo;Zhang, Xiufang;Yang, Sheng*;Li, Yuan;Qing, Zhihe;...

期刊： Analytical Chemistry,2017年89(8):4587-4594 ISSN：0003-2700

通讯作者： Yang, Ronghua;Yang, Sheng

作者机构： [Li, Yuan; Zhang, Xiufang; Zheng, Jing; Yang, Ronghua; Zhou, Yibo; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Li, Yuan; Zhang, Xiufang; Zheng, Jing; Yang, Ronghua; Zhou, Yibo; Li, Jishan] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.

通讯机构： [Yang, Ronghua] H;[Yang, S; Yang, RH] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410114, Hunan, Peoples R China.

摘要： It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results consistently demonstrate that the mutually dependent upgeneration of H2S and NO are present in living biosystems, indicating that this molecular probe would provide a powerful approach to elucidate the chemical foundation for the anfractuous cross-talk between the NO and H2S signaling pathways in biology. © 2017 American Chemical Society.

语种： 英文

作者： Yi, Cuiping*;Li, Yan;Ping, Junai

期刊： Journal of Texture Studies,2017年48(5):386-391 ISSN：0022-4901

通讯作者： Yi, Cuiping

作者机构： [Li, Yan; Yi, Cuiping] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Ping, Junai] Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Jinzhong 030006, Shanxi, Peoples R China.

通讯机构： [Yi, Cuiping] C;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.

关键词： germination;pasting properties;protein;sorghum;texture

摘要： The changes in sorghum [Sorghum bicolor (L.) Moench] proteins during germination and the resultant effects on the physicochemical properties of sorghum flour were studied using non-germinated grains as a control. Results showed that flour obtained from germinated sorghum grains had lower protein levels, higher protease levels, and higher free amino nitrogen content compared with the control. There was an increase in the albumin and globulin protein fractions and a decrease in kafirin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that this decrease was the result of a decrease in γ-kafirins, while microscopy found that a continuous protein network was formed. Following the germination-associated protein changes, the viscosities of sorghum paste decreased with germination from a peak viscosity value of 1,324 rapid visco units (RVU) down to 727 RVU in white sorghum (WS), and from 1,549 RVU to 1,295 RVU in red sorghum (RS). The hardness of the sorghum gels was significantly enhanced after germination, with WS increasing from 1,640 g to 5,374 g and RS from 970 g to 5,529 g. Thus, the study revealed that germination decreased the viscosity of sorghum paste and increased the strength of sorghum gel by changing the content and structure of sorghum protein, making it possible to design new foods that require thickening and gelling using germinated sorghum. Practical applications: Germination triggers the protease system of sprouting seeds, leading to the breakdown of proteins into simpler forms that decrease the viscosity of sorghum paste and improve the strength of sorghum gel, allowing the use of germinated sorghum to design new foods that require thickening and gelling. © 2016 Wiley Periodicals, Inc.

语种： 英文

作者： Zhou, Yi*;Wu, Yiwei;Li, Yanhua;Liu, Yuhuan;Yang, Luyue;...

期刊： Ceramics International,2016年42(10):12482-12489 ISSN：0272-8842

通讯作者： Zhou, Yi

作者机构： [Yang, Luyue; Liu, Yuhuan; Luo, Qinsheng; Zhou, Yi; Wu, Yiwei; Wang, Lipin; Liu, Hai; Li, Dandan] Changsha Univ Sci & Technol, Dept Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.;[Li, Yanhua] Changsha Univ, Hunan Key Lab Appl Environm Photocatalysis, Changsha 410022, Hunan, Peoples R China.

通讯机构： [Zhou, Yi] C;Changsha Univ Sci & Technol, Dept Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China.

关键词： Urchin-likeTiO(2);Reduced graphene oxide;Composite;Photocatalyst

摘要： 3D urchin-like TiO2(UT)/reduced graphene oxide (RGO) composite was fabricated via two processes. Pure UT was first synthesized by solvothermal reaction and UT/RGO(UTG) composite was then prepared by hydrothermal reaction. The urchin-like morphology of as-prepared material was confirmed by SEM and TEM. XRD analysis confirmed the presence of RGO and demonstrated that both pure UT and UTG composite consist of a rutilephase. FT-IR, Raman spectroscopy and XPS characterization demonstrated that graphene oxide was successfully reduced to RGO and RGO well combined with UT. The UV–visible spectrum of UTG composite showed strong absorption in the visible light region (i.e., a blue shift was observed). The UTG composite exhibited larger specific surface area than pure UT (and much larger than P25) owing to the addition of RGO. The formation of Ti–O–C bond possibly separated the photo-generated carriers and transferred the photoelectrons to RGO quickly because of the high electron mobility of RGO, thereby hindering the recombination process. Moreover, experimental results indicated that the UTG composite showed high efficiency in photodegradation of RhB. © 2016 Elsevier Ltd and Techna Group S.r.l.

语种： 英文

作者： Luo, Rongxing;Li, Yinhui;Zhou, Qifeng;Zheng, Jing*;Ma, Dandan;...

期刊： ANALYST,2016年141(11):3224-3227 ISSN：0003-2654

通讯作者： Zheng, Jing;Yang, Ronghua

作者机构： [Luo, Rongxing; Tang, Pinting; Zheng, Jing; Yang, Ronghua; Zheng, J; Li, Yinhui; Ma, Dandan; Zhou, Qifeng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zheng, J; Yang, RH] H;[Yang, Ronghua] C;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.

摘要： In this communication, we propose a gold nanorod-based SERS nanotracker to monitor the local pH change during photothermal therapy (PTT). The dynamic SERS analysis indicated that the local pH of the lysosome in living cells increased during the PTT which would be helpful to understand the cellular metabolic processes and further facilitate the photosensitizer screening and optimization.

语种： 英文

作者： Tao, Jia;Zhao, Peng;Li, Yinhui*;Zhao, Wenjie;Xiao, Yue;...

期刊： Analytica Chimica Acta,2016年918:97-102 ISSN：0003-2670

通讯作者： Yang, Ronghua;Li, Yinhui

作者机构： [Yang, Ronghua; Tao, Jia] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Xiao, Yue; Zhao, Wenjie; Yang, Ronghua; Tao, Jia; Li, YH; Li, Yinhui; Zhao, Peng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Tao, Jia] S China Univ Technol, Coll Chem Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China.

通讯机构： [Yang, Ronghua] C;[Li, YH; Yang, RH] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

关键词： Electrochemical sensors;Fluoride ion;SWCNTs;Spiropyran

摘要： In the past decades, numerous electrochemical sensors based on exogenous electroactive substance have been reported. Due to non-specific interaction between the redox mediator and the target, the instability caused by false signal may not be avoided. To address this issue, in this paper, a new electrochemical sensor based on spiropyran skeleton, namely SPOSi, was designed for specific electrochemical response to fluoride ions (F-). The breakage of Si-O induced by F- based on the specific nucleophilic substitution reaction between F- and silica would directly produce a hydroquinone structure for electrochemical signal generation. To improve the sensitivity, SPOSi probe was assembled on the single-walled carbon nanotubes (SWCNTs) modified glassy carbon electrode (GCE) through the π-π conjugating interaction. This electrode was successfully applied to monitor F- with a detection limit of 8.3 × 10-8 M. Compared with the conventional F- ion selected electrode (ISE) which utilized noncovalent interaction, this method displays higher stability and a comparable sensitivity in the urine samples. © 2016 Elsevier B.V.

语种： 英文